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Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  Inhibitors were assayed against purified hMMP-1, hMMP-2, hMMP-3, hMMP-8, hMMP-9, and hMMP-13 using an enzyme assay based on cleavage of the quenched fluorogenic peptide substrate. The increase in fluorescence due to cleavage of the substrate was measured with a fluorescence microplate reader. The Ki values were calculated by nonlinear regression analysis using the percent inhibition, and Km values of the substrates for each MMP.
Affinity data for this assay
 

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Last update November 1, 2007
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