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Assay Method Information

Assay Name:  Homogeneous Time-resolved Fluorescence (HTRF) Assay
Description:  Kinase activity was measured in a homogeneous assay in a 96-well format. Detection was performed by HTRF using an EuK-labelled anti-phospho(S21)-GSK3 alpha and streptavidin-linked XL665 fluorophore which bound to the biotin moiety on the substrate peptide.
Affinity data for this assay
 

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Last update November 1, 2007
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