Assay Method Information |
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| Kinase Inhibition Assay |
Description: | The enzyme was assayed with a biotinylated peptide substrate and test compounds in the presence of 5 uM ATP/[gamma-33P]ATP in a streptavidin coated FlashPlate (Perkin-Elmer, Boston, MA). Inhibition of the enzymatic activity due to the compound was measured by observing a reduced amount of 33P-gamma-ATP incorporated into the immobilized peptide relative to untreated controls. Linear regression analysis of the percent of inhibition by the test compound was used to calculate IC50 values. |
Affinity data for this assay | |
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