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Assay Method Information

Assay Name:  Enzyme Assay and Determination of the Inhibition Constants.
Description:  Ki values were obtained from human purified enzyme. All assays were run in microtiter plates. Plates were read for 30 min at 405 nm. Rates were determined for the controls (no inhibitor) and for the inhibitors. Percent enzyme activity was determined from these rates and used in the following formula to determine Ki: Ki=(1000)(inhibitor concentration)/{[(Km + S) - (S)(ACT)]/[(ACT)(Km)] -1}, where S is the substrate concentration and ACT is the % enzyme activity for inhibitor.
Affinity data for this assay
 

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Last update November 1, 2007
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