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Assay Method Information |
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| | Kinase Assay and Binding Constant Measurement |
| Description: | Competition binding assay was used to measure the interaction between the phage-tagged kinase, immobilized competitive ligand, and unlinked test compound. The binding constant of test compound (Kd) is calculated by the equation: Kd=(Kd(probe)/(Kd(probe)+[probe])) x [test]1/2. Kd(probe) is the bnding constant for the interaction between kinase and immobilized ligand.[probe] is the concentration of immobilized ligand. [test]1/2 is the concentration of free test compound in the midpoint of transition. If [probe] is <<Kd (probe), Kd = [test]1/2, and the Kd is independent of the affinity between the kinase and immobilized ligand. |
| Affinity data for this assay | |
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