Assay Method Information |
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| In Vitro Kinase Assay |
Description: | The kinase activity was determined by incubation of enzyme and its substrate, and test compound, in the presence ATP/[gamma-32P] ATP. After incubation, the reaction was stopped, and the 32P-phosphorylated substrate was determined by scintillation counting. |
Affinity data for this assay | |
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