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| | PDE Enzyme Inhibitor Assays |
| Description: | PDE5 or PDE6 catalytic activity was monitored by measuring the hydrolysis of [3H]-cGMP to [3H]-GMP using a scintillation proximity assay (SPA). [3H]-GMP was captured by the SPA beads, and quantified on a TopCount plate reader (Packard, Meridien, CT). Radioactivity units were converted to percent activity of an uninhibited control (100%) and plotted against inhibitor concentration, and inhibitor IC50 values were obtained using the Fit Curve Microsoft Excel extension. |
| Affinity data for this assay | |
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