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| | TF-VIIa Assay |
| Description: | Recombinant human Factor VIIa and soluble tissue factor were added to a 96-well assay plate containing substrate and inhibitor in reaction buffer. The rate of hydrolysis of the substrate was measured by monitoring the reaction at 405 nm for the release of p-nitroaniline. All compounds were assayed in duplicate at seven concentrations. Percent inhibition at each concentration was calculated from OD405nm value from the experimental and control sample. IC50 values were calculated from a four-parameter logistic regression equation. |
| Affinity data for this assay | |
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