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| | Phosphodiesterase (PDE) Inhibition Assay |
| Description: | Phosphodiesterase activities were assayed in the presence of inhibitor compounds. Measurement takes advantage of the selective binding of 5-AMP or 5-GMP (and not cAMP or cGMP) to yttrium silicate beads with embedded scintillant. Hydrolysis is quantified by reading in a scintillation counter (Trilux, Wallac). The cAMP and cGMP concentrations used were far below the Km of PDE. Thus, the IC50s obtained are good approximation of Ki. |
| Affinity data for this assay | |
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