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Assay Method Information

Assay Name:  Akt Inhibition Assay
Description:  Purified recombinant human Akt1, Akt2, and Akt3 proteins were monitored for kinase activity in the presence or absence of Akt inhibitors in a 96-well format. Detection was done by homogeneous time-resolved fluorescence (HTRF) using a europium-labeled Phospho (S21)-GSK3a antibody, and streptavidin-linked XL665 fluorophore which binds to the biotin moiety on the substrate peptide.
Affinity data for this assay
 

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Last update November 1, 2007
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