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| | ATF-2 Phosphorylation Assay |
| Description: | In vitro substrate phosphorylation using purified recombinant JNK, was incubated with substrate, and test compounds in the presence of 5uM ATP/ [gamma-33P] ATP. 33P incorporation into substrate was measured with a Perkin-Elmer Micro-Beta plate counter, IC50 values were calculated from dose-response curves. |
| Affinity data for this assay | |
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