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| | In Vitro BACE1 Activity Assay |
| Description: | For BACE activity assay using recombinant MBP-APP_NFEV substrate, BACE was incubated with purified MBP-APP_NFEV substrate in reaction buffer containing inhibitor compound. The cleavage product was analyzed by western blot using neo-epitope specific antibody against N-terminal fragment of APP with the “NFEV” mutants cleaved at the beta-site. |
| Affinity data for this assay | |
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