Assay Method Information |
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| Enzyme Inhibition Assay |
Description: | The activity of the test enzyme was determined spectrophotometrically by monitoring the change in absorbance at 340 nm, which is due to the oxidation of NADPH or the reduction of NAD+ catalyzed by ALR2. The determination of the IC50 values was performed by using linear regression analysis of the log dose response curve. |
Affinity data for this assay | |
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