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Assay Method Information |
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| | Enzyme Inhibition Assay |
| Description: | Enzymes were incubated with inhibitors at eight inhibitor concentrations bracketing the Ki, prepared by serial dilution along with control lacking the inhibitor. Substrate was added, and initial rate of substrate hydrolysis were determined using a UV/MAX Kinetic Microplate Reader. Ki values were determined with BatchKi or true Ki values with DYNAFIT. |
| Affinity data for this assay | |
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