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Assay Method Information

Assay Name:  In Vitro Aldose Reductase Inhibition Assay
Description:  The activity of the enzyme was determined spectrophotometrically by monitoring the change in absorbance at 340 nm, which is due to the disappearance of NADPH. Inhibition by test compound was measured as the percentage decrease in the rate of NADPH oxidation as compared to a sample without test compound. The determination of the IC50 values was performed by using linear regression analysis of the log dose response curve.
Affinity data for this assay
 

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Last update November 1, 2007
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