Assay Method Information |
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| Enzyme Inhibition Assay |
Description: | The activity of the test enzyme was determined spectrophotometrically by monitoring the change in absorbance at 340 nm, which is due to the disappearance of NADPH. Percent inhibition is calculated on the basis of the enzyme activity in the presence or absence of compound. The IC50 is calculated using SAS release 6.10 and a four-parameter logistic regression of data. |
Affinity data for this assay | |
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