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Assay Method Information

Assay Name:  Inhibitory Activity Measurement of Bovine Cathepsin B.
Description:  Inhibitory activities (IC50, concentration of 50% inhibition) of compounds for bovine CB in vitro were determined by monitoring the cleavage of fluorogenic substrate Z-Phe-Arg-NMec. Fluorescence of the liberated aminomethylcoumarin was measured in a fluorimeter. Furthermore, the second order rate constant k2 (M-1s-1) for inactivation of bovine CB was determined as a function of incubation time.
Affinity data for this assay
 

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Last update November 1, 2007
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