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| | Inhibitory Activity Measurement of Bovine Cathepsin B. |
| Description: | Inhibitory activities (IC50, concentration of 50% inhibition) of compounds for bovine CB in vitro were determined by monitoring the cleavage of fluorogenic substrate Z-Phe-Arg-NMec. Fluorescence of the liberated aminomethylcoumarin was measured in a fluorimeter. Furthermore, the second order rate constant k2 (M-1s-1) for inactivation of bovine CB was determined as a function of incubation time. |
| Affinity data for this assay | |
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