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Assay Method Information |
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| | Akt Kinase Assay |
| Description: | The kinase assay uses His-Akt1 and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidin-coated FLASH plates. After reaction, the 33P-phosphopeptide captured on the FLASH plates was measured with a TopCount Packard Instruments gamma counter (Packard Instruments, Boston, MA). |
| Affinity data for this assay | |
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