Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Chk1 Ki determination |
| Description: | For Ki determinations a matrix of inhibitor and substrate concentrations were tested. Inhibitor concentrations were tested from four times IC50 with 0.6-fold dilutions over 7 concentrations, as well as a final reaction with no inhibitor present. Substrate concentrations ranged from 0.5Km to 8Km. An estimate of the Vmax and Km of Chk1 was obtained in the absence of inhibitor, using the Michaelis-Menten steady state equation. For each inhibitor, an initial suggested competitive or non-competitive mode was inferred from analysis of double reciprocal Lineweaver-Burk plots. Then the experimental data were fitted alternatively to a competitive and a non-competitive equation using a 3D non-linear regression. To confirm which model fitted the data best, several statistical parameters were checked. Ki values were then obtained from the relevant model. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||