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Assay Method Information

Assay Name:  Esterase Assay
Description:  Initial rates of 4-nitrophenyl acetate hydrolysis catalyzed by different CA isozymes were monitored spectrophotometrically at 400 nm. A molar absorption coefficient of 18400 M-1 cm-1 was used for the 4-nitrophenolate formed by hydrolysis. Ki values were obtained from Easson-Stedman plots using a linear regression program, from at least three different assays. Standard errors were of 5-10%.
Affinity data for this assay
 

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Last update November 1, 2007
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