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Assay Method Information

Assay Name:  RAF Kinase Assay
Description:  The biochemical activity of compound was determined by incubation with Raf kinases and substrate MEK-1 in the presence of 1-10 uM ATP/ [gamma-32P] ATP. After incubation, the reaction was stopped and the phosphorylated MEK-1 was harvested by filtration, and filter- bound radioactivity was quantified by beta-plate counter.
Affinity data for this assay
 

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Last update November 1, 2007
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