Assay Method Information |
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| PDK1 Kinase Activity Assay |
Description: | In vitro PDK1 assay using purified enzyme, was incubated with substrate PDKtide, and test compounds in the presence of ATP/ [gamma-32P] ATP. 32P incorporation was measured with a scintillation counter. IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from 32P labeled ATP to the substrate. |
Affinity data for this assay | |
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