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Assay Method Information

Assay Name:  Binding Assay
Description:  In order to examine binding ability of the agonists of the present invention to androgen receptors, the following in-vitro experiment was performed. African green monkey kidney fibroblast-like cell line, COS-7 (ATCC, #CRL-1651) was seeded in a 48-well plate at a density of 2.5104 cells/well, and cultured for 24 hours. Then, a plasmid hAR-mixed medium was added thereto. The transfected cell line was treated with 1 nM of [3H]MIB and 0.110,000 nM of SARM derivatives, and then allowed to react for 2 hours. Thereafter, the cells were lysed and the amount of [3H]MIB bound to the intracellular androgen receptors was measured using a radiation dosimeter.
Affinity data for this assay
 

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Last update November 1, 2007
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