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Assay Method Information

Assay Name:  Chk1 Enzymatic Assay
Description:  In vitro Chk1 enzymatic assay using purified enzyme, was incubated with substrate, and test compounds in the presence of 10 uM ATP/ [gamma-32P] ATP. 32P incorporation was measured with a scintillation counter. IC50 values were determined by a least-squares fit to the equation, CPM = Vmax X(1 - ([I]/(IC50 + [I]))) + nonspecific binding. Ki,app = IC50/(1 + [ATP]/Km).
Affinity data for this assay
 

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Last update November 1, 2007
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