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Assay Method Information |
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| | BACE-1 Enzymatic Assay |
| Description: | BACE-1 activity was measured at pH 5 using the FS1 FRET substrate. Compounds were preincubated with recombinant BACE-1 for 20 min before adding substrate. Cleavage of substrate was monitored on a Polarstar fluorometer (Ex 390 nm/Em 520 nm) for 20 min, reading fluorescence at 1 min intervals to determine the rate of the reaction (Vi) at each inhibitor concentration, [I]. Ki was determined from the curve fit of an equation to the graph of [I] vs Vi. |
| Affinity data for this assay | |
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