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Assay Method Information

Assay Name:  Continuous Spectrophotometric Assay
Description:  Proteolytic cleavage of the ester linkage between the Nva (L-norvaline) and the chromophore (PAP) was monitored for change in absorbance at 370 nm. Initial velocities were determined using linear regression and kinetic constants were obtained by fitting the data to the Michaelis-Menten equation.
Affinity data for this assay
 

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Last update November 1, 2007
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