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| | VLA-4/VCAM-1 binding assay |
| Description: | The 4B4 cells expressing VLA-4 were distributed into each well of a 96-well culture plate for 2 days. Compound and Eu-labeled Human VCAM-1/Fc Chimera were then added to the well. After the reaction, the plates were measured Eu fluorescence using a time-resolved fluorometer (DELFIA Research fluorometer, model 1234-001; Perkin-Elmer Inc.). The concentration of compound required for 50% inhibition in the assay was determined. |
| Affinity data for this assay | |
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