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Assay Method Information |
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| | Determination of the Inhibition Constant KI |
| Description: | The oxidation of NADPH was monitored at 340 nm using a Molecular Devices SpectraMax Plus 96-well microtiter plate reading spectrophotometer. Plots were generated using SigmaPlot version 8.0 software and fit to the Michaelis-Menten equation for competitive inhibition. |
| Affinity data for this assay | |
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