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Assay Method Information

Assay Name:  Determination of IC50
Description:  Dihydrofolate reductase was assayed with no inhibitor and with a series of concentrations of inhibitors to allow for a range of inhibition from 10 to 90%. At least three concentrations were required for calculation. Semilogarithmic plots of the data showed a normal sigmoidal plot for most inhibitors. These plots were converted to straight lines by converting percent inhibition to probit values which were plotted versus the log of the drug concentration. The data were fit by least-squares linear regression. The 50% inhibitory concentration (IC50) is the concentration at which the probit value is 5.0.
Affinity data for this assay
 

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Last update November 1, 2007
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