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Assay Method Information

Assay Name:  In Vitro Inhibition Assay
Description:  Enzyme activity assays were performed by monitoring the rate of enzyme-dependent NADPH consumption at an absorbance of 340 nm over a period of several minutes. After an incubation period of 5 min in the presence or absence of the inhibitor, the reaction was initiated with the addition of dihydrofolate. The reactions were performed a minimum of four times to determine the average rate and 50% inhibitory concentration values.
Affinity data for this assay
 

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Last update November 1, 2007
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