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| | Luciferase Reporter Assay |
| Description: | Activities of the test compounds to inhibit androgen receptor mediated transcription induced by DHT were evaluated using stable transfected AR/CHO#3 cells plated onto 96-well luminoplates. After cells were lysed, cell extracts were prepared for luciferase assay. The luciferase activity was measured with a ML3000 luminometer. AR antagonist activities were calculated. The concentration of compounds showing 50% AR antagonist activity, the IC50 values, were obtained through nonlinear analysis using the statistical analysis system (SAS). |
| Affinity data for this assay | |
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