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Assay Method Information

Assay Name:  Competitive Radioligand Displacement Assay
Description:  The Ki values were determined by the application of the Cheng-Prusoff equation: Ki = (IC50 x Kd)/(Kd+[L]) where [L] is the concentration of [3H]MIB (1 nM ) and Kd is the dissociation constant of the [3H]MIB determined in the saturation analysis (Kd=0.19 nM for MIB).
Affinity data for this assay
 

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Last update November 1, 2007
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