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Assay Method Information

Assay Name:  HDAC Enzyme Activity Assay
Description:  For the enzyme assay, the enzyme fraction was added to fluorescent substrate in the presence of test compounds, and the mixture was incubated for additional 30 min. The released aminomethyl coumarin (AMC) was measured using a fluorescence plate reader. The 50% inhibitory concentrations (IC50) were determined as means with SD calculated from at least three independent dose response curves.
Affinity data for this assay
 

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Last update November 1, 2007
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