Assay Method Information |
|
| Estrogen Receptor Binding Assay. |
Description: | Relative binding affinities were determined by a competitive radiometric binding assay using [3H]estradiol as tracer, and purified full-length human ER. Hydroxyapatite was used to adsorb the receptor-ligand complexes, and free ligand was washed away. The binding affinities are expressed as relative binding affinity (RBA) values with the RBA of estradiol set to 100%. The values given are the average +/- range or SD of two to three independent determinations. Estradiol binds to ERalpha with a Kd of 0.2 nM and to ERbeta with a Kd of 0.5 nM. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |