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Assay Method Information

Assay Name:  Competition-Based Ligand Binding Assay.
Description:  Ligand binding was determined using a scintillation proximity assay with streptavidin-coated polyvinyltoluene scintillation beads (Amersham) and biotinylated receptor. Receptor-bound [3H]-E2 was determined by scintillation counting (Perkin-Elmer). The IC50 values were calculated using a four-parameter logistic equation.
Affinity data for this assay
 

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Last update November 1, 2007
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