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| | Estrogen Receptor Binding Assay and Cell-Based Transcriptional Assay |
| Description: | The binding affinities were determined by a competitive radiometric binding assay using [3H]estradiol as tracer. The Kd for 3H-estradiol was determined by saturation binding to ER receptors. The IC50 values for compounds were converted to Ki using Cheng-Prusoff equation. The functional activity and selectivity was measured using a transcription assay in the cotransfected human prostate cancer PC3/ER (alpha or beta)-ERE cell line, and EC50 values were determined by computer fit to a concentration-response curve. |
| Affinity data for this assay | |
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