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Assay Method Information |
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| | Hsp90 Fluorescence Polarization Binding Assay |
| Description: | Compounds were assayed over a threefold dilution series in a 96-well plate format to determine IC50 values. The reaction was allowed to proceed with gentle rocking for 3 h at room temperature. Fluorescence polarization was measured on a Wallac Envision with a 480 nM excitation filter and 535 nM polarized emission filters. |
| Affinity data for this assay | |
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