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Assay Method Information

Assay Name:  Radioligand Labeled Binding Assay and [35S]GTP-gamma-S Binding Assay
Description:  Log IC50 values for each test compound were determined from nonlinear regression analysis of data collected from two independent experiments performed in duplicates (40 independent experimental values) using GraphPad Prizm 4 software (GraphPad, San Diego, California). The inhibition constant (Ki) was calculated from the antilogarithmic IC50 value by the Cheng and Prusoff equation. Opioid agonist functional activities of test compounds, measured as EC50 values were obtained from [35S]GTP-gamma-S binding assay.
Affinity data for this assay
 

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Last update November 1, 2007
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