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| | Enzyme Inhibition Assay |
| Description: | Enzyme assay was conducted in 384-well microplates format. Activity was monitored by measuring increase in fluorescence (excitation @320 nm, emission @405 nm) over a 60 minute time course using a BMG Polarstar Galaxy fluorescent plate reader. Compound IC50 values were determined using 10-pt concentration ranges. Rates of substrate hydrolysis were determined from time courses and converted to percent inhibition. Percent inhibition was plotted versus compound concentration and fit to the four parameter logistic equation by non-linear regression using Prism Graphpad software. |
| Affinity data for this assay | |
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