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| | Radioligand Binding Assay and [35S] GTP-gamma-S Binding Assay |
| Description: | A scintillation proximity assay was used for radioligand competition and saturation binding assays. Nonspecific binding was determined in the presence of 1 uM PGD2. Binding activity was determined by using a 1450 Microbeta scintillation counter (Wallac, UK). Ki values were calculated by using the Cheng-Prusoff equation, and represent the average of at least three independent dose-response experiments. EC50/IC50 values were obtained from GTP-gamma-S binding assay, which measures antagonist activity of compounds in the presence of 80 nM PGD2. |
| Affinity data for this assay | |
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