Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Enzyme Inhibition Assay |
| Description: | The enzyme reaction was initiated by adding fluorescence peptide substrate to reaction mixture containing enzyme and test compounds. The enzyme activity was determined by following the change of fluorescence released by the hydrolysis of the substrates, using a fluorescent spectrophotometer (Hitachi F4500) with excitation wavelength of 360 nm and emission at 480 nm. Hydrolysis rates were recorded in presence of six to seven different concentrations of inhibitor. The Ki values were determined by Dixon plots from two sets of data with different concentrations of substrate. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||