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| | PPAR FRET Assay |
| Description: | Activity was assessed by measuring the human PPAR ligand binding domain with the co-activator protein. Europium labeled anti-GST antibody, streptavidin-labeled APC, biotin-labeled co-activator protein, and PPAR-GST were incubated at room temperature in buffer in the presence or absence of test compound. Following 2 h incubation, fluorescence was measured at emission wavelengths of 615 or 655 nm, with excitation at 340 nm. |
| Affinity data for this assay | |
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