Assay Method Information |
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| Enzyme Inhibition Assay |
Description: | The ability of the compounds prepared in this study to inhibit purified recombinant human IDO was evaluated with a steady state spectrophotometric assay using microtiter plates and a plate reader (Tecan Infinite M200). All assays were performed in triplicate, and the resulting double-reciprocal plots were analyzed by weighted linear-least-squares fits to the data. |
Affinity data for this assay | |
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