Assay Method Information |
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| PNP Inhibition Assay |
Description: | PNP activity was monitored by absorbance change in a coupled assay. In the assay, inosine was converted to hypoxanthine, and then hypoxanthine was converted to uric acid by including 60 milliunits of xanthine oxidase (buttermilk, Sigma Corp.) in the reaction mixtures, and by following uric acid formation at 293 nm which gave 12.9 mM-1 cm-1. |
Affinity data for this assay | |
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