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Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  To assess the effect of test compounds on histone deacetylase enzyme function in Vitro, a fluorometric assay was performed using HDAC, which incubated with fluorophore-conjugated substrate. Quantitative measurements were obtained in real time on a Varioskan microplate reader (Thermo), in the presence of increasing concentrations of the inhibitor. Data were normalized to a control reaction in the presence of an equal volume of DMSO. Each measurement represents the arithmetic mean of three independent experiments. Increasing concentrations of all compounds resulted in a dose dependent inhibition of HDAC.
Affinity data for this assay
 

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Last update November 1, 2007
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