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Assay Method Information

Assay Name:  Human 11beta-HSD1 Scintillation Proximity Assay (SPA)
Description:  The 11beta-HSD1 enzyme assay was carried out in the replica plates of the compounds in reaction buffer containing substrate mixture [3H]-cortisone/NADPH. Reactions were initiated by the addition of the enzyme. After incubation, the amount of the product, [3H]-cortisol, captured on the anticortisol antibody-coated SPA beads was determined in a microplate liquid scintillation counter. Kinetic constants were calculated using the Microsoft Excel integrated application XLfit (Version 5.3.0.19, ID Business Solutions Ltd.) using the sigmoidal dose-response model 205, which is based on the nonlinear curve fitting based on Levenberg-Marquardts algorithm.
Affinity data for this assay
 

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Last update November 1, 2007
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