BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Radioligand Binding Assay and Functional [35S]GTP-gamma-S Binding Assay
Description:  Ligand displacement assays were performed on CHO cells membranes expressing hH3R. Retained radioactivity was determined by liquid scintillation counting. Nonspecific binding was determined in the presence of 1 uM thioperamide. The Ki values were calculated based on an experimentally determined appropriate Kd value according to Cheng and Prusoff. EC50 is a measure of functional property of compound on [35S]GTP-gamma-S binding to membranes expressing H3R receptors.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.