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| | Enzyme Inhibition Assay |
| Description: | CE inhibition was determined using a spectrophotometric multiwell plate assay with o-NPA as a substrate. The rate of change in absorbance at 420 nm was measured at 15 sec intervals for 5 min and compared to wells containing no inhibitor. Compounds that demonstrated 50% reduction in CE activity were subsequently evaluated in detail. Further analysis of inhibitors was performed in condition that inhibitor concentrations ranged from 1 pM to 100 uM. Data were fitted to the equation. Examination of curve fits to identify the best model for enzyme inhibition. Ki values were then calculated from the equation predicted by Prism to be the best fit for the experimental data. |
| Affinity data for this assay | |
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