Assay Method Information |
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| Time-Dependent Inhibition Assay |
Description: | For the time-dependent inhibition studies, COX enzyme was incubated with test compounds for 20 min and then analyzed for remaining COX activity by treatment with [1-14C]arachidonic acid 30 sec at 37°C. All IC50 values are average determinations from two independent experiments. |
Affinity data for this assay | |
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