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Assay Method Information

Assay Name:  Time-Dependent Inhibition Assay
Description:  For the time-dependent inhibition studies, COX enzyme was incubated with test compounds for 20 min and then analyzed for remaining COX activity by treatment with [1-14C]arachidonic acid 30 sec at 37°C. All IC50 values are average determinations from two independent experiments.
Affinity data for this assay
 

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Last update November 1, 2007
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