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Assay Method Information |
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| | In Vitro Ligase Activity Assay |
| Description: | The assay was performed using a 40-bp double-stranded DNA substrate carrying a single-strand nick between bases 22 and 23. The reaction products were resolved electrophoretically. The autoradiograms were developed, and the extent of ligation was measured. The IC50 values were determined by plotting the relative ligation activity vs inhibitor concentration, and fitting to equation Vi/Vo=IC50/(IC50+[I]) using GraphPad Prism. Vo and Vi represent the rate of ligation in the absence and presence of inhibitor. |
| Affinity data for this assay | |
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